Pages: 94-100DOI: 10.56228/JART.2022.SP115

Date of Publication: 31-Dec-2022

Highly Efficient Conversion Biomass of Saccharum munja for Cellulases and Xylanase Production to Ethanol Repression by Newly Isolated Trichoderma atroviride AD-130

Author: Seema Devi , Meenakshi Suhag, Joginder Singh, Anil Dhaka

Category: Agricultural Engineering

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Abstract:

In this study the filamentous fungi Trichoderma atroviride AD-130 was evaluated for production of cellulases as well as xylanase. An attempt has been made to optimize the cultural and nutritional conditions for cellulases and xylanase production by Trichoderma atroviride AD-130 under submerged fermentation. The lignocellulosic biomass of Saccharum munja was used as carbon source for cellulases and xylanase production. Whole fermentation process was carried out in 250 mL Erlenmeyer flasks with agitation speed of 170rpm. The maximum titers of cellulases (FPase 1.01 U mL-1, CMCase 2.69 U mL-1, b-glucosidase 0.82 U mL-1) and xylanase (82.99 U mL-1) were obtained on 5th and 4th day respectively when Trichoderma atroviride AD-130 was grown at initial medium having pH 6.0 at 30__degreesignC using 2.0% (w/v) Saccharum munja biomass as substrate.

Keywords: Trichoderma atroviride AD-130, Saccharum munja, Cellulase, Xylanase, Ethanol.